Journal: British journal of pharmacology

Article Title: Regulator of G-Protein Signalling 4 (RGS4) negatively modulates nociceptin/orphanin FQ opioid receptor signalling: Implication for l-Dopa-induced dyskinesia.

doi: 10.1111/bph.15730

Figure Lengend Snippet: FIGURE 7 AT-403 inhibited the L-Dopa-stimulated pGluR1 phosphorylation in the striatum of dyskinetic rats. Western blot representative images (upper panel) and quantification (lower panel) of pGluR1 (a) and total GluR1 (b) in the striatum of 6-OHDA hemilesioned, L-Dopa-naïve or dyskinetic rats. Dyskinetic rats were treated with the NOP receptor agonist AT-403 (0.03 mgkg1, s.c.) or vehicle and, 10 min later, challenged with L-Dopa (6 mgkg1, i.p.). The experimental RGS4 chemical probe CCG-203920 (10 mgkg1, i.p.) or vehicle were administered 5 min before AT-403. Values are mean ± SEM of six rats per group. *P < 0.05, different from unlesioned striatum (Student's t-test, two-tailed for unpaired data)

Article Snippet: Proteins were then transferred onto polyvinyldifluoride membrane, blocked for 60 min with 5% non-fat dry milk in 0.1% Tween20 Tris-buffered saline and incubated overnight at 4 C with anti-Thr202/Tyr204-phosphorylated ERK1/2 (pERK) rabbit monoclonal antibody (Merck Millipore, Cat# 05-797R, RRID:AB_1587016, 1:1000), anti-ERK1/2 (totERK) rabbit polyclonal antibody (Merck Millipore Cat# 06-182, RRID:AB_310068, 1:5000), anti-phospho-Ser845 GluR1 (pGluR1) rabbit polyclonal antibody (PhosphoSolutions, Cat# p1160-845, RRID:AB_2492128, 1:1000), anti-glutamate receptor 1 (totGluR1) rabbit polyclonal antibody (Merck Millipore Cat# AB1504, RRID:AB_2113602, 1:1000), nloaded from https://bpspubs.onlinelibrary.w iley.com /doi/10.1111/bph.15730 by C ochrane Israel, W iley O nline L ibrary on [23/06/2023].

Techniques: Phospho-proteomics, Western Blot, Two Tailed Test